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1- Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran
2- Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran ,Mohammadzadeh@basu.ac.ir
3- Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
4- Department of Microbiology and Immunology, Faculty of Veterinary Science, Tehran University, Tehran, Iran
2- Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran ,
3- Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
4- Department of Microbiology and Immunology, Faculty of Veterinary Science, Tehran University, Tehran, Iran
Abstract: (111 Views)
Abstract
Background: Biofilm formation in Staphylococcus aureus (S. aureus), mediated by the ica operon, is a key virulence factor. This study examined how different glucose-supplemented broth culture media influence biofilm production and ica gene expression in S. aureus.
Methods: The phenotypic ability to adhere to a polystyrene surface and to produce slime layer were evaluated using microtiter plate test (MtP) and Congo red tube test, respectively. Using PCR, the presence of intercellular adhesion (ica) locus in S. aureus strains was confirmed and subsequently, quantitative real-time RT-PCR was performed to investigate transcription of icaA in various media including Tryptic soy broth (TSB), Brain-heart infusion broth (BHIB), (Nutrient broth) NB and (Muller-Hinton broth) MHB contained 0, 0.25, 0.5, 1 and 2% glucose.
Results: Our results showed that although all of the studied strains adhered to the wells of polystyrene microtiter plates, the optimum rate of biofilm formation was observed for TSB medium contained 1% glucose, but biofilm formation was not significantly different in NB, MHB and BHIB media. Supplementation of all media with 1% glucose led to the highest production of biofilm formation and in all media transcription of icaA was increased with glucose addition to one present.
Conclusions: The results of the present study indicated that TSB medium supplemented with 1% glucose was the most appropriate medium for evaluation of biofilm formation by S. aureus isolates.
Background: Biofilm formation in Staphylococcus aureus (S. aureus), mediated by the ica operon, is a key virulence factor. This study examined how different glucose-supplemented broth culture media influence biofilm production and ica gene expression in S. aureus.
Methods: The phenotypic ability to adhere to a polystyrene surface and to produce slime layer were evaluated using microtiter plate test (MtP) and Congo red tube test, respectively. Using PCR, the presence of intercellular adhesion (ica) locus in S. aureus strains was confirmed and subsequently, quantitative real-time RT-PCR was performed to investigate transcription of icaA in various media including Tryptic soy broth (TSB), Brain-heart infusion broth (BHIB), (Nutrient broth) NB and (Muller-Hinton broth) MHB contained 0, 0.25, 0.5, 1 and 2% glucose.
Results: Our results showed that although all of the studied strains adhered to the wells of polystyrene microtiter plates, the optimum rate of biofilm formation was observed for TSB medium contained 1% glucose, but biofilm formation was not significantly different in NB, MHB and BHIB media. Supplementation of all media with 1% glucose led to the highest production of biofilm formation and in all media transcription of icaA was increased with glucose addition to one present.
Conclusions: The results of the present study indicated that TSB medium supplemented with 1% glucose was the most appropriate medium for evaluation of biofilm formation by S. aureus isolates.
Keywords: Biofilm formation, Staphylococcus aureus, Culture media, glucose, quantitative real-time RT-PCR
Research Article: Research Article |
Subject:
Microbiology
Received: 2024/05/23 | Accepted: 2025/07/26
Received: 2024/05/23 | Accepted: 2025/07/26
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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