Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
2538-4449
10.61186/mlj
en
jalali
1397
6
1
gregorian
2018
9
1
12
5
online
1
fulltext
fa
Detection of fim, pap, sfa and afa Adhesin-Encoding Operons in Escherichia coli Strains Isolated from Urinary Tract Infections
مروري
Review Article
<div><strong>ABSTRACT</strong><br>
<strong> Background and objectives:</strong> Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is <em>Escherichia coli</em>. Uropathogenic <em>E.coli </em>(UPEC) strains have a number of specific virulence factors, which can worsen UTIs. This study was performed to detect <em>fim</em>, <em>pap</em>, <em>sfa</em> and <em>afa</em> genes among <em>E.coli</em> strains isolated from UTIs.<br>
<strong> Methods:</strong> A total of 100 <em>E. coli</em> isolates from patients with UTI was collected between June and December 2015 from Mosavi and Sayyad Shirazi hospitals in Gorgan, Iran. All bacterial isolates were identified via standard biochemical testing and Gram straining. Presence of the genes was assessed by polymerase chain reaction.<br>
<strong>Results: </strong>The frequency of the <em>fim</em>, <em>pap</em>, <em>sfa </em>and <em>afa </em>genes was 100%, 79%, 69% and 8%, respectively. All isolates contained at least one virulence gene. Prevalence of multiple adhesion genes was 6% for all genes and 65% for three genes (<em>fim</em>, <em>pap</em> and <em>sfa)</em> together. In addition, the frequency of the <em>fim</em> gene was significantly higher than that of the other genes (P<0.0001).<br>
<strong>Conclusion:</strong> The results of this study indicate the high prevalence of virulence factors that can enhance pathogenicity of <em>E. coli</em>. Therefore, these factors could be used as diagnostic markers or vaccine targets.<br>
<strong>Keywords: </strong>Virulence factors, Urinary tract infection, Uropathogenic <em>Escherichia coli</em>.</div>
Virulence factors, Urinary tract infection, Uropathogenic Escherichia coli
10
15
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-538&slc_lang=fa&sid=1
Mahsa
Yazdi
100319475328460014550
100319475328460014550
No
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Majid
Bouzari
bouzari@sci.ui.ac.ir
100319475328460014551
100319475328460014551
Yes
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Ezzat Allah
Ghaemi
100319475328460014552
100319475328460014552
No
Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran