en فیزیولوژی ورزشی Sport Physiology تحقيقي Original Paper <p style="margin: 0px; text-align: justify; text-justify: kashida; text-kashida: 0%;"><strong>ABSTRACT</strong><br> <strong>Background and Objectives:</strong> Exercise is a strong physiological stimulus that can affect apoptosis in the lungs by altering a number of extracellular and intracellular signaling pathways. The present study examined effects of regular aerobic exercise and vitamin D on expression of Bcl-2, Bax and caspase-3 in lung tissues of male rats exposed to hydrogen peroxide.<br> <strong>Methods:</strong> Forty-eight adult male Wistar rats were randomly assigned into six groups of eight, including 2 x H 2 O 2, 2H 2 O 2, Vit D (2HD), 2H2O2 + Regular Exercise Training (2HE), 2H2O2 + D3 + E (2HDE) ; Dimethyl sulfoxide (DMSO) and control (C). Subjects in the training groups performed aerobic exercise for eight weeks. Bax, Bcl-2, caspase-3 expression in the lung tissues was measured using RT-PCR.<br> <strong>Results:</strong> Bcl-2 expression in the exercise (P = 0.004) and vitamin D (P = 0.006) groups increased significantly compared to the control groups. Bax, Bcl-2 and caspase-3 expression was significantly lower in the exercise group and vitamin D supplementation group compared to the control group. On the other hand, concurrent exercise and vitamin D significantly reduce Bax expression but had no significant effect on Bcl-2 and caspase-3 expression.<br> <strong>Conclusion: </strong>Our results demonstrate that regular aerobic exercise along with vitamin D supplementation may play a role in reducing apoptosis in lungs following severe oxidative stress.<br> <strong>Keywords:</strong> Apoptosis, Bcl-2, Bax, Caspase3, Aerobic Exercise, Vitamin D<br> &nbsp;<br> &nbsp;</p> <p style="margin: 0px; text-align: justify; text-justify: kashida; text-kashida: 0%;"><span style='margin: 0px; line-height: 115%; font-family: "Times New Roman","serif"; font-size: 14pt;'></span></p> Apoptosis, Bcl-2, Bax, Caspase3, Aerobic Exercise, Vitamin D 44 49 http://mlj.goums.ac.ir/browse.php?a_code=A-10-768-1&slc_lang=en&sid=1 somayeh ramezani ramezani.s20@gmail.com 100319475328460018828 100319475328460018828 No Dept. of Exercise Physiology, School of Physical Education and Sport Sciences, Central Tehran Maghsoud peeri m.peeri@iauctb.ac.ir 100319475328460018829 100319475328460018829 Yes Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran,IRAN Mohammad Ali Azarbaijani ali.azarbayjani@gmail.com 100319475328460018830 100319475328460018830 No physical education-azad university-Tehran-iran Firoozeh Dehghan fir_dhn@yahoo.com 100319475328460018831 100319475328460018831 No Professor of Tehran University, Physiology