en ميکروب شناسي Microbiology مروري Review Article <div><strong>Background and objectives: </strong>Industrial wastewater is worldwide health concern. Microorganisms present in the environment have an important role in the biodegradation of lipids, fats and proteins from wastewater. In this regard, microbial lipases and proteases are interesting research targets because of high stability, broad substrate specificity, high yields and availability. In this study, we analyze sequences encoding lipase of <em>Pseudomonas putida</em> and subtilisin of <em>Bacillus subtilis </em>for generation of a new recombinant protein for degradation of environmental contaminations caused by lipids and proteins.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods: </strong>In this study, sequences of the genes encoding lipase and subtilisin were obtained from GenBank. To predict the 3D structure of the protein, modeling was carried out. The prediction of secondary structure, tertiary <em>structure</em> and solvent accessibility was carried using bioinformatics tools including I-TASSER, GoR4 and ExPasy.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results: </strong>The lipase-subtilisin fusion protein was well-characterized by bioinformatical studies with appropriate spatial and secondary structures. The protein had appropriate hydrophilicity, biological half-life and thermal and acidic stability. The codon optimization was performed appropriately.</div> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion: </strong>Overall, the bioinformatical analysis of the designed protein showed that the recombinant lipase-subtilisin protein has a stable structure both in vitro and in vivo, a negative normalized B-factor and lipolytic and proteolytic activities, which makes it suitable for treatment of lipid and protein contaminations. Pseudomonas putida, Bacillussubtilis, Lipase, subtilisin, Fusion protein, Bioinformatic analysis 23 27 http://mlj.goums.ac.ir/browse.php?a_code=A-10-589-3&slc_lang=en&sid=1 Neda Gharagozloo Hesari n.gharagozlue@gmail.com 100319475328460018793 100319475328460018793 No Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran Davoud Esmaeili esm114@gmail.com 100319475328460018794 100319475328460018794 No Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran and Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Taher Mohammadian tmohammadian@qodsiau.ac.ir 100319475328460018795 100319475328460018795 Yes Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran Mohammad Hasan Shahhosseini shahhosseiny@yahoo.com 100319475328460018796 100319475328460018796 No Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran Atousa Ferdosi esmaeili 14@yahoo.com 100319475328460018797 100319475328460018797 No Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran