en باکتری شناسی bacteriology Research Article Research Article <span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Urinary tract infection (UTI) is one of the most prevalent bacterial diseases worldwide. <i>Escherichia coli</i> is a well-known etiological agent of UTI. The emergence and spread of metallo-beta-lactamase (MBL)-producing <i>E. coli</i> is a serious threat to public health.<br> This study aimed to investigate the antibiotic resistance pattern and prevalence of MBL-producing <i>E. coli</i> isolated from UTI.<br> <b>Methods:</b> From January 2020 to June 2021, 1200 urine specimens were collected from patients suspected of having UTI. Antibiotic susceptibility testing was carried out by the disk diffusion method. The prevalence of MBL (<i>bla</i>VIM, <i>bla</i>IMP, <i>bla</i>SPM, and <i>bla</i>NDM) genes was determined by the polymerase chain reaction (PCR) method.<b><span style="font-size:8.0pt"></span></b><br> <b>Results:</b> The highest susceptibility was observed against amikacin (96%) and gentamicin (95%). The isolates were mostly resistant against ampicillin (72%) and cephalothin (60%). All carbapenem-resistant isolates were MBL-positive. Based on the results of PCR, 75% of the isolates were <i>bla</i>NDM-positive.<b><span style="font-size:8.0pt"></span></b><br> <b>Conclusion</b>: Resistance to some antibiotics, such as ampicillin and cephalothin, was high, and their prescription must be restricted. The prevalence of MBL-producing isolates was not high; however, due to the high level of resistance against other antibiotics, continuous monitoring of MBL-producing isolates is highly essential.<span style="font-size:8.0pt"></span></span></span> Escherichia coli,Urinary tract infection,Metallo-beta lactamase 19 22 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1178-1&slc_lang=en&sid=1 Fatemeh Rashedi fatemerashede74@gmail.com 100319475328460026650 100319475328460026650 No School of Medicine, Zabol University of Medical Sciences, Iran Zahra Yazdanpour yazdanpp106@gmail.com 100319475328460026651 100319475328460026651 No Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Iran Farzad Khademi k_farzad@yahoo.com 100319475328460026652 100319475328460026652 No Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Iran Hamid Vaez hamidvaez@hotmail.com 100319475328460026653 100319475328460026653 Yes Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Iran