en باکتری شناسی bacteriology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Background</span></span></b><span style="line-height:200%"><span new="" roman="" times="">: Urinary tract infection (UTI) is one of the most common bacterial infections. <i>Staphylococcus saprophyticus</i> is a common Gram-positive bacterium that causes uncomplicated UTIs in women. The present study aimed to study the drug resistance pattern and phenotypic and genotypic variation of <i>S. saprophyticus</i> isolates from women with UTI in Gorgan, northern Iran.</span></span><span dir="RTL" lang="FA"><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Methods</span></span></b><span style="line-height:200%"><span new="" roman="" times="">: This study was performed from May 2018 to September 2020. During this time,&nbsp; &nbsp;35 <i>S. saprophyticus</i> strains were isolated from patients with UTI. The antimicrobial patterns of the isolates were determined by a conventional method. Phenotypic criteria such as pigment production, mannitol fermentation, urease production, and <i>16SrRNA</i> gene valuation were studied.</span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Results</span></span></b><span style="line-height:200%"><span new="" roman="" times="">: All isolates were sensitive to nitrofurantoin, gentamicin, and linezolid. <i>S. saprophyticus </i>isolates showed the highest level of resistance to penicillin (85.7%) and erythromycin (51.4%). A variation was detected among <i>S. saprophyticus</i> isolates in terms of pigment production i.e. about 51.4% showed yellow pigment in Muller Hinton agar, and 62.9% of the isolates were able to ferment mannitol sugar. Of 11 isolates that were sequenced for the <i>16SrRNA</i> gene, only two isolates showed different patterns.</span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Conclusion</span></span></b><span style="line-height:200%"><span new="" roman="" times="">: Nitrofurantoin and trimethoprim-sulfamethoxazole are the antibiotics of choice for the treatment of UTI caused by <i>S. saprophyticus</i> in the study area. Due to the phenotypic and genotypic differences among <i>S. saprophyticus</i> isolates, typing of <i>S. saprophyticus</i> at the subspecies level is recommended.<b> </b></span></span></span></span></span></span></div> Staphylococcus saprophyticus,Urinary tract infection,Drug Resistance, Microbial, 33 38 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1315-1&slc_lang=en&sid=1 Maryam Rafiee sefidrooze@yahoo.com 100319475328460031974 100319475328460031974 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Alijan Tabarraei alijant@yahoo.com 100319475328460031975 100319475328460031975 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Mahsa Yazdi mahsayazdi92@gmail.com 100319475328460031976 100319475328460031976 No Department of Biology, Faculty of Sciences, University of Isfahan, Iran Alireza Mohebbi alirezaa2s@gmail.com 100319475328460031977 100319475328460031977 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Ezzat Allah Ghaemi eghaemi@yahoo.com 100319475328460031978 100319475328460031978 Yes Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran