en باکتری شناسی bacteriology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Typhoid fever, a severe febrile illness prevalent in tropical regions, remains a significant cause of mortality. Drug-resistant pathogens have spurred research into medicinal plants as alternative treatments. While previous studies have focused on the inhibitory effects of <i>Moringa oleifera</i> leaf extract, limited attention has been given to <i>M. oleifera</i> seed oil and its effects on Gram-negative bacteria. This study aimed to evaluate the anti-typhoidal activity of <i>M. oleifera</i> seed oil against both typed and clinical <i>Salmonella typhi </i>isolates.<br> <b>Methods:</b> The anti-typhoidal activity of <i>M. oleifera</i> seed oil was assessed using agar diffusion and disk diffusion methods. Phytochemical screening of the seed oil was also conducted to identify its constituents.<br> <b>Results:</b> <i>M. oleifera</i> seed oil exhibited negligible or weak inhibitory effects on clinical and typed <i>S. typhi</i> strains (p>0.05), in contrast to the substantial inhibition observed with commercial antibiotics. Ciprofloxacin demonstrated the highest zones of inhibition (&ge;30 mm) against <i>S. typhi</i>, while tetracycline showed the least pronounced inhibition (&le;16 mm). Phytochemical screening of <i>M. oleifera</i> seed oil identified terpenoids, cardiac glycosides, saponins, tannins, and flavonoids, with terpenoids being the most abundant and flavonoids the least abundant.<br> <b>Conclusion</b>: <i>M. oleifera</i> seed oil lacks significant therapeutic potential for the treatment of typhoid fever. Its antibacterial constituents are minimal or absent, resulting in insignificant inhibition against <i>S. typhi.</i><i></i></span></span></div> Typhoid Fever, Moringa Oleifera, Antibiotics, Salmonella Typhi, Phytochemicals, Seed oil 7 10 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1795-1&slc_lang=en&sid=1 Sikiru Kayode Abdulahi kayode.abdulahi2@gmail.com 100319475328460032110 100319475328460032110 Yes The Federal University of Technology Akure, P.M.B. 704, Akure, Ondo State, Nigeria