Medical Laboratory Journal

en Impact assessment of Sclareol on endoplasmic reticulum stress in KATO III human gastric carcinoma cancer cell lines ژنتیک انسانی Human Genetics Research Article Research Article Introduction and objectives: The main goals of chemotherapy are to increase apoptosis or cell death and to stop the cell cycle in cancer cells. One of the very important factors inducing apoptosis in cancer cells is to increase the stress of the endoplasmic reticulum system. In this study, the authors intended to investigate the effect of Sclareol on the ATF6 pathway as well as the effect of two other sub-genes, VEGF and E2F1, associated with the UPR pathways. Materials and methods: The target population in this research was KATO III human gastric carcinoma cancer cells, which were purchased from the Pasteur Institute of Iran, and they were cultured in the cell culture laboratory until their number reached at least 100,000 cells/well. The cells were treated with concentrations of 0, 20, 40, 60, 80 and 100 μM of Sclareol for 5 hours. Analyzing the expression of the genes- expressed in the research objectives- derived from gastric cancer cells before and after treatment with Sclareol was performed using Quantitative Real Time-PCR from the spheres of cell lines cultured in suspension. Results: ATF-6 gene expression significantly increased in genes with doses of 20, 40 and 60 μM of Sclareol (P<0.0001). On the other hand, it was shown that the level of VEGF gene expression was significantly reduced in genes with doses of 20, 40 and 60 μM of Sclareol (P<0.001). Also, there was a significant increase in E2F1 gene expression in doses of 20, 40 and 60 μM of Sclareol (P<0.003).   Gastric Cancer- The Endoplasmic Reticulum System Stress- Sclareol - KATO III Gastric Cancer Cells 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1835-1&slc_lang=en&sid=1 Mojtaba Ghaffarian Gabalou benzbmw142@gmail.com 100319475328460033163 100319475328460033163 No Department of Molecular Genetics, Ah.C., Islamic Azad University, Ahar, Iran Seyed Ali Rahmani rahmaniseyedali@yahoo.com 100319475328460033164 100319475328460033164 Yes Department of Molecular Genetics, Ah.c , Islamic Azad University, Ahar, Iran Changiz Ahmadizadeh Dr_ahmadizadeh@yahoo.com 100319475328460033165 100319475328460033165 No Department of Microbiology, Ah.c , Islamic Azad University, Ahar, Iran Rasoul Sharifi rasoulsharifi.sci@gmail.com 100319475328460033166 100319475328460033166 No Department of biochemistry, Ah.c , Islamic Azad University, Ahar, Iran