fa تحقيقي Original Paper <div> <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objectives: </strong>Diabetes is one of the most common endocrine disorders, which is associated with changes in testicular tissue. The present study investigated therapeutic and prophylactic properties of bitter orange (<em>Citrus aurantium</em>) juice on testicular tissue and spermatogenesis process.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> Forty streptozotocin-induced diabetic Wistar rats aged three months with mean weight of 170-200 g were divided into 4 groups including 1) control group, 2) diabetic control group, 3) diabetic group receiving 100mg/kg <em>C. aurantium</em> extract and 4) diabetic group receiving 200 mg/kg <em>C. aurantium</em> extract. The extract was administered to the rats for 56 days by gavage. After this period, the rats were anesthetized with ether and then their testes were fixed in 10% formalin for sample preparation. The testicular tissue was examined by haematoxylin and eosin staining under a light microscope with 10 and 40 magnifications. The mean number of Leydig and Sertoli cells, spermatogonia, spermatocytes and spermatids were calculated.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> A significant decrease was observed in mean weight of left testis in diabetic rats compared to that of controls (P&le;0.05). The mean weight of testes showed no significant difference in diabetic rats treated with 200 mg/kg of extract compared with the control group. Diabetes reduced the number of spermatogonia, spermatocytes, spermatids and Sertoli cells. The number of cells increased significantly in the diabetic group receiving 200 mg/kg of extract. The spermatocytes and spermatids in both groups treated with the extract increased significantly.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> This study shows the positive effect of bitter orange extract on complications of diabetes in testicular tissue. Therefore, this extract could be used for therapeutic purposes.</p> </div> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> diabetes, bitter orange juice, spermatogenesis, testis, rat</p> diabetes, bitter orange juice, spermatogenesis, testis, rat. 43 48 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-429&slc_lang=fa&sid=1 Leili Komeilifard leilikomeilifard@yahoo.com 100319475328460012503 100319475328460012503 No Department of Biology, Jahrom Beranch Vahid Hemayat khahjahromi 100319475328460012504 100319475328460012504 Yes Department of Biology, Jahrom Beranch