fa تحقيقي Original Paper <div> <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective:</strong> Several studies have</p> <p>shown the antimicrobial activity of lactobacilli against <em>Salmonella enterica</em> (serotype<em> typhimurium</em>). The aim of this study was to evaluate the inhibitory potential of metabolites produced by probiotic culture of <em>Lactobacillus casei</em> against <em>S. typhimurium</em> and its impact on <em>S. typhimurium</em> motility and biofilm formation.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> In order to evaluate the impact of the metabolites, <em>L. casei</em> cell-free culture supernatant (CFCS) was collected by centrifugation of <em>L. casei</em> secondary cultures. Effectiveness of the CFCS against Salmonella was evaluated by the well-diffusion method. In addition, in vitro effect of this treatment on motility in Swarm agar and biofilm formation by the bacteria was investigated.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> Inhibition zone diameters of <em>S. typhimurium</em> were 0.83 and 12.1 mm at concentrations of 50 and 100 &mu;l of Lactobacillus CFCS against the log4 of <em>S. typhimurium</em>, respectively. Moreover, CFCS treatment inhibited the motility and biofilm formation by Salmonella. Concentrations of 5% and 10% were determined as the minimum inhibitory concentrations for motility and biofilm formation by <em>S. typhimurium</em>. Furthermore, effectiveness of the CFCS against Salmonella was dose-dependent (P<0.05).</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> <em>L. casei</em> CFCS is able to inhibit the growth, motility and biofilm formation in <em>S. typhimurium</em>.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> Anti-Bacterial, <em>Lactobacillus Casei</em> Metabolites, Phenotypic Characteristics of <em>S. typhimurium</em></p> </div> Anti-Bacterial, Lactobacillus Casei Metabolites, Phenotypic Characteristics of S. typhimurium 7 13 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-431&slc_lang=fa&sid=1 Hossein Naghili h.naghili@gmail.com 100319475328460012444 100319475328460012444 Yes PhD of Food Hygiene Hossein Tajik 100319475328460012445 100319475328460012445 No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine Mojtaba Raeisi 100319475328460012446 100319475328460012446 No Department of Health, Faculty of Health, Hadi Ghasem Mahdi 100319475328460012447 100319475328460012447 No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine Mehran Moradi 100319475328460012448 100319475328460012448 No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine Majid Amin zare 100319475328460012449 100319475328460012449 No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine Touraj Mehdizadeh 100319475328460012450 100319475328460012450 No Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine Hasan Hasanzadazar 100319475328460012451 100319475328460012451 No Department of Food Hygiene and Quality Control Fardin Hariri 100319475328460012452 100319475328460012452 No Doctor of Veterinary Medicine and Master of public health