fa تحقيقي Original Paper <div> <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective:</strong> Although antibiotics are commonly used for treatment of infectious diseases, these treatments are often associated with several problems such as unwanted side effects and resistance to antibiotics. The aim of this study was to evaluate the antibacterial effect of aqueous and alcoholic extracts of <em>Nasturtium officinale</em> on <em>Escherichia coli, Salmonella typhimurium, Staphylococcus aureus</em> and <em>Listeria monocytogenes</em>.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods: </strong>All experiments were performed using the well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the aqueous and alcoholic extracts of <em>N. officinale</em> against the pathogenic bacteria studied were determined by microdilution method.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> In the well diffusion method, <em>S. aureus</em> and <em>L. monocytogenes</em> were the most sensitive bacteria with MIC of 8 &mu;g/ml, while <em>E. coli</em> and <em>S. typhimurium</em> were the most resistant bacteria to the aqueous and alcoholic extracts. In addition, the inhibitory activity of the alcoholic and aqueous extracts of <em>N. officinale </em>was higher against gram-positive bacteria compared to gram-negatives. The lowest MIC (6.25 &mu;g/ml) and MBC (12.5 &mu;g/ml) of the plant extract were against <em>S. aureus</em>.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> The aqueous and alcoholic extracts of <em>N. officinale </em>affect the growth of gram-positive bacteria (<em>S. aureus</em> and <em>L. monocytogenes</em>) but not the gram-negatives (<em>S. typhimurium</em> and <em>E. coli</em>). These extract could be used for treatment of infections.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> <em>Nasturtium Officinale</em>, Aqueous Extract, Alcoholic Extract, Antibacterial Effect.</p> </div> Nasturtium Officinale, Aqueous Extract, Alcoholic Extract, Antibacterial Effect. 49 53 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-432&slc_lang=fa&sid=1 Saeid Farshbaf Derhami 100319475328460012485 100319475328460012485 No Department of Microbiology, Faculty of Basic science Biology Mehdi Ghiami Rad 100319475328460012486 100319475328460012486 No Department of Microbiology, Faculty of Basic science Biology Razzagh Mahmoudi 100319475328460012487 100319475328460012487 Yes Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine