Showing 4 results for Antibodies
Azadi F, Hedayat Mofidi, M , Ali Arab, A , Hedayat Mofidi, H , Khorasaninjad, R , Ghasemi-Kebria, F , Roshandel, Gh ,
Volume 9, Issue 3 (9-2015)
Abstract
Background and Objective: Helicobacterpylori(HP) is the most common bacterial infection in the world and it may be associated with chronic gastritis, pepticulcer disease, and gastricadenocarcinoma. The aim of this study was to determine the prevalence of HP in student of Golestan University of Medical Sciences (GOUMS).
Material and Methods: This cross-sectional study was conducted on students ofGOUMS in 2012. Anti-HP IaG and IgA were assessed byELISA method.
Results: The participants recruited were 287 in that 101 (35.2%) were male and the rest female. Of 287 , 239 (83.3%) were positive for HP. The proportion of men was 91.1% and women 79% (P =0.03). There was no significant relationship between HP infection and variables such as age, blood group , place of residence, nativity, and level of education.
Conclusion: The prevalence of H. pylori infection in the students of Golestan University of Medical Sciences is high.
Mohsen Dashti, Afsane Bahrami, Mohammad Hadi Sadeghian, Seyyede Fatemeh Shams, Ahmad Ashjaee, Zahra Arianpour ,
Volume 11, Issue 4 (7-2017)
Abstract
ABSTRACT
Background and Objectives: Blood transfusion may induce some adverse effects on receivers. Some methods such as antibody screening and cross matching have been suggested to reduce the risk of transfusion complications. However, these methods require commercial antibody screening kits that may also need special equipment. The aim of this study was to introduce a new method for antibody screening that does not require a commercial kit, and could be used in any transfusion laboratory.
Methods: We examined 350 samples that contained alloantibody and 350 control samples without the antibody. A solution containing two O+ and one O- samples were used instead of screening cells.
Results: Sensitivity and specificity of the method were 73.32% and 45.15%, respectively. Positive predictive value and negative predictive value were 58.33% and 63.88%, respectively.
Conclusion: Our new method can be used in basic hematology laboratories with some modifications.
Keywords: Antibodies, Antigens, Coombs test.
Mana Zakeri, Elham Alimoradi, Effat Seyyedhashemi, Shayan Marhamati, Vahid Tajari, Hamidreza Joshaghani,
Volume 17, Issue 2 (3-2023)
Abstract
Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, caused by abnormal innate and adaptive immune responses. Anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) are reliable biomarkers for diagnosing SLE. Here, we aimed to investigate the serum levels of anti-dsDNA and ANA antibodies, their diagnostic utilities, and their relationship with disease activity and clinical/laboratory manifestations in patients with suspected.
Methods: We evaluated the plasma levels of ANA and anti-dsDNA antibodies in all individuals with suspected SLE (n=668) who had been referred to rheumatology clinics in Gorgan, Iran. The level of antibodies as well as C3, C4, and CH50 were determined using commercially available enzyme-linked immunosorbent assay kits.
Results: The mean level of ANA and anti-dsDNA antibodies differed significantly between the ANA-positive and ANA-negative groups (p<0.001). However, there was no significant difference in the mean values of C3 (p=0.233), C4 (p=0.415, and CH50 (p=0.482) between the two groups. Moreover, there was a significant positive correlation between ANA and anti-dsDNA levels (p<0.001, r=0.50).
Conclusion: Our findings indicate that anti-dsDNA levels are higher in ANA-positive individuals, and there may be a positive correlation between ANA and anti-dsDNA levels. It is recommended to evaluate the diagnostic and prognostic values of ANA and anti-dsDNA antibodies in future studies.
Adrian Yong Sing Lee,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Autoimmune liver diseases (ALD) are a heterogeneous group of disorders affecting the hepatobiliary system and are characterized by specific autoantibodies. These are routinely measured in diagnostic laboratories using commercial line immunoblot (LIB) assays. However, the ordering characteristics and diagnostic performance of this test have not been extensively evaluated. This study aims to examine the performance of the ALD LIB in a single diagnostic laboratory.
Methods: A retrospective, cross-sectional audit of 12 months of data was performed at the Institute of Clinical Pathology and Medical Research diagnostic laboratory (Westmead Hospital, Australia). Patients referred for an ALD-LIB were included. Medical notes were reviewed to ascertain the clinical diagnoses of patients. Patients who had at least one positive ALD autoantibody on LIB were defined as “blot-positive” and compared with “blot-negative” patients. The performance of the ALD-LIB was assessed through the calculation of diagnostic sensitivities and specificities.
Results: There were 611 patients included over the 12-month period. Sixty-four of these patients (10%) were blot-positive. These patients were more likely to be female, to have other ALD-associated autoantibodies, and to have lower alkaline phosphatase (ALP) levels compared with blot-negative patients. An ALD diagnosis or systemic autoimmune disease was more likely to be identified in blot-positive patients. Finally, the LIB demonstrated a high negative predictive value for an ALD diagnosis in this patient cohort.
Conclusion: This real-world analysis of the laboratory’s ALD-LIB provided insights into the ordering characteristics and performance of this assay in patients referred for testing. When combined with other ALD investigations, the ALD-LIB is a useful adjunct in the evaluation of patients with suspected ALD.
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