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Showing 2 results for Salmonella Typhi
In vitro Susceptibility Study of Salmonella typhimurium Mutant to Stress Factors
Ania Ahani Azari , Taghi Zahraei Salehi , Bahar Nayeri Fasaei ,
Volume 10, Issue 6 (11-2016)
Abstract

ABSTRACT

        Background and Objective: In this study, we compared the susceptibility of Salmonella typhimurium phoP mutant and its parent to stress conditions that the pathogen may encounter in a host.

        Methods: For this purpose, we used the phoP deletion mutant constructed in our previous study. In order to test the in vitro susceptibility of the mutant to stress factors, the effect of acidic pH, heat, bile salts and polymyxin on growth of the mutant was examined. Then, minimum inhibitory concentration and minimum bactericidal concentration of bile salts and polymyxin were determined. Salmonella typhimurium 14028 was used as the parent strain.

         Results: The mutant was highly susceptible to bile salts and polymyxin in comparison with the parent strain, but no difference was observed in their susceptibility to acid and heat.

          Conclusion: This study confirms the role of the phoP in resistance of Salmonella to polymyxin and bile salts. Mutation in the phoP leads to susceptibility of the mutant to bile and cationic antimicrobial peptides.

         Keywords: In vitro, Mutant, Salmonella typhimurium, Disease Susceptibility.


Comparison of antityphoidal efficacy of Moringa oleifera seed oil to antibiotics against Salmonella typhi
Sikiru Kayode Abdulahi ,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Typhoid fever, a severe febrile illness prevalent in tropical regions, remains a significant cause of mortality. Drug-resistant pathogens have spurred research into medicinal plants as alternative treatments. While previous studies have focused on the inhibitory effects of Moringa oleifera leaf extract, limited attention has been given to M. oleifera seed oil and its effects on Gram-negative bacteria. This study aimed to evaluate the anti-typhoidal activity of M. oleifera seed oil against both typed and clinical Salmonella typhi isolates.
Methods: The anti-typhoidal activity of M. oleifera seed oil was assessed using agar diffusion and disk diffusion methods. Phytochemical screening of the seed oil was also conducted to identify its constituents.
Results: M. oleifera seed oil exhibited negligible or weak inhibitory effects on clinical and typed S. typhi strains (p>0.05), in contrast to the substantial inhibition observed with commercial antibiotics. Ciprofloxacin demonstrated the highest zones of inhibition (≥30 mm) against S. typhi, while tetracycline showed the least pronounced inhibition (≤16 mm). Phytochemical screening of M. oleifera seed oil identified terpenoids, cardiac glycosides, saponins, tannins, and flavonoids, with terpenoids being the most abundant and flavonoids the least abundant.
Conclusion: M. oleifera seed oil lacks significant therapeutic potential for the treatment of typhoid fever. Its antibacterial constituents are minimal or absent, resulting in insignificant inhibition against S. typhi.

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