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Showing 4 results for Sequencing
Prevalence of Malassezia species isolated from skin of patients with seborrheic dermatitis reffered to Tonekabon clinics by PCR- Sequencing method
A Raefi, N Nasrollahi Omran, A Nazemi,
Volume 9, Issue 2 (7-2015)
Abstract

Abstract

Background and Objective: Malassezia yeast is considered lipophilic normal flora of human skin and warm-blooded vertebrates. This fungus is an opportunistic pathogen in causing seborrheic dermatitis. In this study, the yeasts isolated from the crust of the patients with seborrheic dermatitis were identified by PCR-Sequencing.

Material and Methods: In this study, 65 samples of the skin of ear, nose and dandruff were cultured in selective medium Sabouraud agar and modified Dixon agar to prevent dehydration. After biochemical tests, ITS1-4 Universal PCR primers were used to determine the species of yeast.  Obtained PCR products were sequenced for the determination and identification of Malassezia species.

Results: Of nine samples obtained from scalp, four were Malassezia globosa, two Malassezia restricta, two Cryptococcus albidus and one Cryptococcus albidus milis.

Conclusion: The results of Malassezia globosa and Malassezia Restericta are very similar with those in studies elsewhere.

Keywords: Malassezia, Sequencing, Seborrheic Dermatitis, Tonekabon


Probable Role of Bilophila wadsworthia in Appendicle Infection
Mohammad Tabatabaei, Aslam Dehvari, Bita Geramizadeh, Mohammad Hadi Niakan,
Volume 14, Issue 1 (1-2020)
Abstract
ABSTRACT
           Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens.
           Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers.
           Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.
           Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues.
           Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing

Genes Associated with Coma or Recurrent Coma and Role of Next Generation Sequencing in Diagnosis of Disease-Causing Genes
Fatemeh Asadi, Hamedreza Goodarzi, Javad Zahiri, Mojtaba Jafarinia,
Volume 16, Issue 1 (1-2022)
Abstract
Coma is a state of prolonged unconsciousness. Some coma cases result from inherited disorders such as fatty-acid β-oxidation disorder, acute intermittent porphyria (due to mutations in genes CPT I, CPTII and ACADM), urea cycle defects (due to mutation in OTC gene), organic acidurias, mitochondrial diseases and familial hemiplegic migraine (due to mutations in CACNA1A, ATP1A2 and SCN1A). The evaluation of familial cases of coma or sporadic coma can be performed using next generation sequencing (NGS), a high-throughput  sequencing technique that can sequence an entire genome in a single reaction. This technique has been widely applied in the genetic diagnosis of diseases. In this review, we describe some genes associated with coma or recurrent coma and discuss the role of NGS in detection of these genes.  
Investigation of Cryptosporidium oocyst contamination in water from Zabol and Zahedan: A molecular and microscopic analysis
Mansour Dabirzadeh , Reza Shahraki , Mohammadreza Beheshtizadeh , Mahdi Khoshsima Shahraki,
Volume 20, Issue 2 (6-2026)
Abstract
Background: Cryptosporidium is one of the most important protozoan parasites causing waterborne diseases worldwide. The parasite’s oocysts are resistant to conventional water treatment, making molecular detection crucial for identifying contamination sources.
Methods: A total of water samples were collected from different sites in Zabol and Zahedan, southeastern Iran. Microscopic screening was performed after concentration and staining with modified Ziehl-Neelsen and Trichrome methods under 1000× oil-immersion magnification. DNA was extracted from positive samples, and the SSU rRNA gene (~800-900 bp) was amplified by PCR. The resulting products were subjected to enzymatic digestion using AluI and RsaI restriction enzymes, and representative amplicons were sequenced.
Results: Microscopic examination confirmed the presence of Cryptosporidium oocysts in several water samples. PCR amplification successfully produced fragments of the expected size without nonspecific bands. AluI digestion revealed distinct fragment patterns consistent with Cryptosporidium spp., while RsaI showed no cutting sites. Sequence analysis through BLAST showed high identity (≥99%) with C. parvum isolates. The phylogenetic tree constructed using the BLAST distance-tree method grouped the sequence closely with C. parvum, confirming its identity.
Conclusion: Molecular characterization of Cryptosporidium from water samples in southeastern Iran indicated contamination primarily with C. parvum. These findings emphasize the necessity of continuous molecular surveillance to ensure the safety of drinking and recreational waters in the region.

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